This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets proteins for degradation by the 26S proteasome. Recent data suggests that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination . S phase kinase-associated protein 1 (Skp1) is a critical scaffold protein of the Skp1/CUL1/F-box (SCF) E3 ubiquitin ligase protein complex.Studies have also characterized the protein as an RNA polymerase II elongation factor.
